TABLE 1.
Expression vector and gene | Primer
(5′–3′)
|
|
---|---|---|
Forward | Reverse | |
pPROK1 | ||
ahpC | CGCCCGGGAGGAGGAAGAATAGATGTTAGTTACAAAACTTGCC | CCCTGCAGCTACAGCTTGATGGAATTTTCTTTAAGAT |
trx1 | CGCCCGGGAGGAGGAAGAATAGATGAGTCACTATATTG | CCCTGCAGTTAGCCTAAAAGTTTGTTCAATTGC |
trx2 | GCGAATTCAGGAGGAAGAATAGATGTCAGAAATGATTAACGGG | GCAAGCTTCCCCTTACAATAACGCTTTTAGAGC |
trxR | CGCCCGGGAGGAGGAAGAATAGATGATAGATTGCGCGATTATTGG | CCAAGCTTGATTTAATGGTG |
pBluescript-SK | ||
ahpC | TGGAATTCGCCCAATAACGATGAAACAAG | GAGAGCTCCGATTAAAGCTTAATGGA |