Figure 2.

Gut bacteria depletion in HFD fed HLA-DR3 transgenic mice ameliorate EAE. (A) Model diagram representing day of diet initiation, antibiotics treatment, EAE induction and disease scoring in HLA-DR3 mice. 6-8-week-old male HLA-DR3 transgenic mice were placed on a HFD or a normal chow diet for 4 weeks while receiving broad spectrum antibiotics in the drinking water followed by immunization with PLP_91-110/CFA to induce EAE. (B) Comparison of mean clinical scores in mice fed a HFD or normal chow diet and while receiving broad spectrum antibiotics in the drinking water. (C) Cumulative EAE scores from mice as in (A). Data are representative of 4 mice per group. Error bars represent the standard error of the mean for each experimental group. p-value was determined by 2way ANOVA test (B, C).