Figure 4.

Functional investigation of the microbial communities in HLA-DR3 Transgenic Mice Fed with HFD and NC. (A) PCoA representing differentially clustering patterns of HFD, NC and prediet communities using Bray Curtis dissimilarity metrics. Lefse analysis showing functional pathways significantly enriched in (B) HFD compared to NC, (C) HFD compared to prediet conditions and, (D) NC and prediet conditions. Lefse analysis was carried out with lda_cutoff of 3 and wilcoxon_cutoff of 0.01, and kw_cutoff of 0.01 respectively with other parameters remaining default. Functional pathways related to sulfur metabolism, long chain fatty acid synthesis, lipopolysaccharides and vitamin K are represented by “S”, “L”, “LPS” and “K” respectively in (B–D).