Figure 5.

A High Fat Diet results in increase gut permeability. (A) Schematic of experimental design. 6-8-week old male mice were placed on the indicated diet for 8 weeks prior to FITC-dextran permeability assay and FABP2 assay. For FITC-dextran permeability assay, mice on either diet were fasted for 6 hours prior to oral treatment with 4 kDa FITC-dextran. Serum samples were taken 2 hours post-treatment. Sera level of FITC was measured, using a spectrophotometer, as a measurement of intestinal permeability. For FABP2 assay, blood sera samples were analyzed by ELISA as per manufacturer protocol. (B) FABP2 concentrations in HFD and Normal chow mice. (C) 4 kDa FITC-dextran level in HFD and Normal chow mice. Error bars represent the standard error of the mean for each experimental group. p-value was determined by Mann-Whitney test between the experimental groups in (B, C).