Figure 6.

MKK4 knockdown alleviates HS-induced mitochondrial damage in the liver. (a) Changes in mitochondrial membrane potential were examined with JC-1, the fluorescence intensity of red and green was measured, and the ratio was calculated. (b) The level of ATP in the livers of HS rats was measured with a kit. (c) The activities of mitochondrial complex I and complex III in the livers of HS rats were examined. (d) Representative Western blot images and quantification of cytochrome c levels in the cytoplasm and mitochondria. Values are presented as mean ± SD, n = 6 for each group.