TABLE 2.
Expression of the asd promoter clone, asd promoter mutants, and E. coli promoters in E. coli and A. pleuropneumoniaea
| Clone | Promoter structure | Lux activity (RLU/OD520)
|
|
|---|---|---|---|
| E. coli | A. pleuropneumoniae | ||
| pTF86 | None | 0.06 ± 0.04 | 0.007 ± 0.004 |
| asd14 | CTAATTGCATTTTAGTAGAATCCTGTATT | 1,110 ± 87 | 838 ± 40 |
| asd15 | CTAATTGCATTTTAGTAGAAATCCTGTATT | 1,604 ± 244 | 2,387 ± 25 |
| asd | CTAATTGCATTTTAGTAGAAAATCCTGTATT | 4,740 ± 117 | 3,330 ± 166 |
| asd17 | CTAATTGCATTTTAGTAGAAAAATCCTGTATT | 5,783 ± 377 | 484 ± 13 |
| asd18 | CTAATTGCATTTTAGTAGAAAAAATCCTGTATT | 11,584 ± 1,685 | 134 ± 10 |
| pSDbla | TACATTCAAATATGTATCCGCTCATGAGACAAT | 13,204 ± 500 | 261 ± 14 |
| pSDstr | TTTCTTGACACCTTTTCGGCATCGCCCTAAAAT | 14,825 ± 211 | 445 ± 26 |
a
The −35 (left) and −10 (right) sequences are in boldface type.