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. 2022 Sep 24;13:5619. doi: 10.1038/s41467-022-33366-x

Fig. 7. Genetic basis of adaptive traits.

Fig. 7

a When compared to diapause wild type (+pnd/+pnd), a 747 bp deletion can be identified in the 3′-UTR of BmTret1-like in non-diapause pnd. b In embryonic stages at 6, 12, and 18 hours, the expression level of BmTret1-like in homozygotes (pnd/pnd) is significantly lower than that in heterozygotes (+pnd/pnd). Three biological duplications were conducted. Data are shown as mean ± SD. c CRISPR/Cas9 mediated BmTret1-like knockout (KO) in wild type (WT, Dazao) individuals. Three BmTret1-like−/− lines, KO1, KO2, and KO3 contain a 25 bp, 28 bp, and 8 bp deletion in BmTret1-like. The BmTret1-like knockout lines generate non-diapause eggs under 25 °C. The wild-type bivoltine strain (WT, Dazao) generates diapause eggs when the maternal embryos were incubated under 25 °C. d Large insertion and duplication in the L locus and the difference between Wnt1-1 and Wnt1-2. e Large deletion in LC locus. f Expression of Wnt1 in +/+ and LC/+, three biological duplications were conducted. Data are shown as mean ± SD. g Wnt1 expression and phenotype after Wnt1 RNAi (n = 20). CT, control (LC/+). Scale bar, 5 mm. Student’s t test (two-tailed). Data are shown as mean ± SD. Source data are provided as a Source Data file.