Fig. 4. Comparison of putative fusions by DNA-seq and RNA-seq.

a Schema of integrating data obtained by DNA-based next generation sequencing (NGS) OncoPanel, and RNA-sequencing (RNA-seq), to pick out expressed oncogenic fusions. b The candidate fusions detected by each fusion caller from RNA-seq data were aligned with putative oncogenic fusions detected by OncoPanel. Fusions detected by all three fusion callers were noted. Asterisk points to data obtained from the leftover RNA sample from the RNA-based anchored multiplex PCR for targeted NGS. c Circos plots showing discordant reads with ABL1, FGFR1, BRAF, DLG1, or NRG1 detected by RNA-seq.