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. 2022 Sep 24;13:5606. doi: 10.1038/s41467-022-33067-5

Fig. 2. Human adipocyte antigen presentation contributes to adipose tissue inflammation.

Fig. 2

a Visceral (VAT) and b Subcutaneous (SAT) adipocyte gene expression in lean (N = 15) and obese (N = 83) subjects of MHCII-related genes by two-sided independent sample T-test. Source data are provided as a Source Data file. Means for lean subject gene expression are presented as normalized to 1. c Co-culture of CD4 + T cells from human obese subjects in the presence/absence of adipocytes and/or antigen (Copaxone (50 µg/ml)) assessing the % Th1 cells of total CD4 + cells by flow cytometry and IFNγ and IL-2 media levels by ELISA analyzed by student T test (without adjustment for multiple comparisons). d Treg differentiation assay: naïve CD4 + T cells (T) from new donors co-cultured, with or without CD3/CD28 antibodies (Abs) to activated T cells, and with conditioned media (CM: IFNγ and IL-2 high) from the previous experiments in c to determine %Tregs of CD4 + cells analyzed by student T test (without correction for multiple comparisons). See text for abbreviations. Data presented as mean ± SEM. ***p < 0.001; **p < 0.01; *p < 0.05; and †p < 0.10. No replicate samples were utilized.