FIG. 3.

Expression of lasI in response to iron deprivation. Wild-type strain PAO1 was transformed with pPCS223, which contains the lasI::lacZ reporter fusion (50), and then cultured to mid-log or stationary phase in TSB (open bars) or TSB amended with the iron-specific chelator 2,2-dipyridyl (filled bars). The data represent the average of the means of three independent experiments (two cultures per experiment). Error bars, where visible, represent 1 standard error of the three experimental means. Differences between iron treatments for each culture stage are statistically significantly different (P = 0.01).