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Mn-SOD expression in PAO1 biofilms as affected by iron starvation. SOD activity was detected using activity stains as described in Materials and Methods. Cell extracts (50 μg per lane) were obtained from PAO1 biofilms grown for 1 or 2 days in 1/10 TSB containing the iron-specific chelator 2,2-dipyridyl and from 1- and 2-day PAO-JP1 biofilms grown in 1/10 TSB. The data are representative of duplicate experiments, and locations of the Mn-SOD and Fe-SOD bands are as shown.
