Table 2.

Production of 5-HTP by microorganisms

Host	Hydroxylases	Cofactors	Cultivation mode	medium type	Titer (g/L)	Reference
E. coli	Phenylalanine 4-hydroxylase form Chromobacterium violaceum with mutations of L101Y and W180F	BH4	Shake flask; Supplementation of 5 mM L-Trp	-	0.55	Hara et al., 2013 [11]
E. coli	Phenylalanine-4-hydroxylase from Xanthomonas campestris with a mutation of W179F	MH4	Shake flask; Supplementation of 2 g/L L-Trp	M9 minimal medium	1.1–1.2	Lin et al.,. 2014 [16]
E. coli	Aromatic amino acid hydroxylase from Cupriavidus taiwanensis with a mutation of W179F	BH4	Supplementation of 1 g/L L-Trp	Mineral medium	0.55	Mora-Villalobos et al., 2017 [44]
E. coli	Aromatic amino acid hydroxylase from Cupriavidus taiwanensis with mutations of F197 L and E219C	MH4	Fed-batch	Mineral medium	0.962	Mora-Villalobos et al., 2018 [17]
E. coli	Human TPH2 mutant with a deletion of first 145 N-terminal and 24 C-terminal aminoacids (TPH2, NΔ145/CΔ24)	BH4	Fed-batch; Glycerol as carbon source	Mineral medium	5.1	Wang et al., 2018 [12]
E. coli	Truncated human TPH2 (NΔ145/CΔ24)	BH4	Shake flask; Glycerol as carbon source	Mineral medium	1.61	Xu et al., 2020 [43]
E. coli	Truncated human TPH2 (NΔ145/CΔ24) with mutations of E2K, N97I and P99C	BH4	Fed-batch; Glucose as carbon source	Mineral medium	8.58	This study