Table 3.
Strains and plasmids used in this study
| Strains | Characteristics | Source |
|---|---|---|
| E. coli DH5α | Host for cloning | Lab stock |
| E. coli K-12 w3110 | Wild type, starting strain | Lab stock |
| HTP01 | E. coli W3110, ∆tnaA, lacI::PxylF-T7RNAP, mlc::mlc* | This study |
| HTP02 | HTP01, yghX::Ptrc-mtrA-PTPS-SPR | This study |
| HTP03 | HTP02, yjgX::PT7-PCD-DHPR | This study |
| HTP04 | HTP03, pSTV-TM | This study |
| HTP05 | HTP03, pSTV-TM1 | This study |
| HTP06 | HTP03, pSTV-TM2 | This study |
| HTP07 | HTP06, trpLE::Ptrc-trpEfbr | This study |
| HTP08 | HTP07, yjiV::Ptrc-aroGfbr | This study |
| HTP09 | HTP08, ycgH::Ptrc-serAfbr | This study |
| HTP10 | HTP09, mbhA::PM1−12-gdhesi | This study |
| HTP11 | HTP09, mbhA::PM1−30-gdhesi | This study |
| HTP12 | HTP09, mbhA::PM1−46-gdhesi | This study |
| HTP13 | HTP09, mbhA::PM1−37-gdhesi | This study |
| HTP14 | HTP09, mbhA::PM1−93-gdhesi | This study |
| Plasmid | ||
| pGRB | gRNA expression vector | Lab stock [45] |
| pRed-cas9 | Cas9 expression vector | Lab stock [45] |
| pSTV28 | P15A ori, CmR, E.coli expression vector | Lab stock |
| pSTV-TM | pSTV28, PT7-TM | This study |
| pSTV-TM1 | pSTV28, PT7-TM1 (TME2K) | This study |
| pSTV-TM2 | pSTV28, PT7-TM2 (TME2K/N97I/P99C) | This study |