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. 2001 Mar;183(6):2013–2024. doi: 10.1128/JB.183.6.2013-2024.2001

TABLE 1.

Bacterial strains and plasmids used in this work

Strain or plasmid Genotype Relevant phenotype Source or reference
E. coli
 HB101 F proA2 hsdS20 recA1 ara-14 lacY1 galK2 rpsL20 supE44 xyl-5 mtl-1 rB mB Stratagene
 S17.1 thi pro hsdR recA ara RP4::2-Tc::Mu::kan:Tn7 integrated into chromosome rB mB+ Tpr Smr 39
R. sphaeroides
 Ga crt Wild type 40
 Gadc2 crt Δ(cycA::spe) Cyt c2 3
 Gadcy crt Δ(cycY::kan) Cyt cy 17
 Gadc2cy crt, Δ(cycA::spe) Δ(cycY::kan) Cyt c2 Cyt cy 17
 MT001 crt Δ(ccoNO::kan) cbb3-Cox 42
 KD02 crt Δ(cycA::spe) Δ(ccoNO::kan) Cyt c2cbb3-Cox This work
 KD04 crt Δ(cycY::spe) Δ(ccoNO::kan) Cyt cycbb3-Cox This work
 JS100 crt Δ(ctaD::spe) aa3-Cox 36
 KD03 crt Δ(cycY::kan) Δ(ctaD::spe) Cyt cyaa3-Cox This work
 KD05 crt Δ(cycA::kan) Δ(ctaD::spe) Cyt c2aa3-Cox This work
 ME127 crt Δ(ctaD::spe) Δ(ccoNO::kan) cbb3-Cox aa3-Cox 42
 BC17 crt Δ(petABC(fbcFBC)::kan) Cyt bc1 45
Plasmids
 pBSII pBlueScript II KS(+) Ampr Stratagene
 pHP45Ω spe cartridge Sper 31
 pHP45Ω-Km kan cartridge Kanr 9
 pSup202 Suicide plasmid Tetr Cmr Ampr 39
 p4.2 cycY::kan in PstI site of pSup202 This work
 pcycA::spe cycA::spe in PstI site of pSup202 This work
 pKD10.2 2-kb spe cartridge of pHP45Ω in BamHI site of cycY carried by pU2000 (in opposite orientation of cycY) This work
 pU2000 1.8-kb BglII-XbaI R. sphaeroides chromosomal DNA carrying cycY in pBSII KS(+) 51
 pKD11 3 kb cycY::spe fragment in EcoRI site of pSup202 This work
 pSupC2 P2.71::kan Δ(cycA::kan) at StuI site of cycA carried at PstI site of pSup202 8