E. coli |
|
|
|
HB101 |
F proA2 hsdS20 recA1 ara-14 lacY1 galK2 rpsL20 supE44 xyl-5 mtl-1
|
rB− mB−
|
Stratagene |
S17.1 |
thi pro hsdR recA ara RP4::2-Tc::Mu::kan:Tn7 integrated into chromosome |
rB− mB+ Tpr Smr
|
39 |
R. sphaeroides |
|
|
|
Ga |
crt |
Wild type |
40 |
Gadc2 |
crt Δ(cycA::spe) |
Cyt c2−
|
3 |
Gadcy |
crt Δ(cycY::kan) |
Cyt cy−
|
17 |
Gadc2cy |
crt, Δ(cycA::spe) Δ(cycY::kan) |
Cyt c2− Cyt cy−
|
17 |
MT001 |
crt Δ(ccoNO::kan) |
cbb3-Cox−
|
42 |
KD02 |
crt Δ(cycA::spe) Δ(ccoNO::kan) |
Cyt c2−cbb3-Cox−
|
This work |
KD04 |
crt Δ(cycY::spe) Δ(ccoNO::kan) |
Cyt cy−cbb3-Cox−
|
This work |
JS100 |
crt Δ(ctaD::spe) |
aa3-Cox−
|
36 |
KD03 |
crt Δ(cycY::kan) Δ(ctaD::spe) |
Cyt cy−aa3-Cox−
|
This work |
KD05 |
crt Δ(cycA::kan) Δ(ctaD::spe) |
Cyt c2−aa3-Cox−
|
This work |
ME127 |
crt Δ(ctaD::spe) Δ(ccoNO::kan) |
cbb3-Cox− aa3-Cox−
|
42 |
BC17 |
crt Δ(petABC(fbcFBC)::kan) |
Cyt bc1−
|
45 |
Plasmids |
|
|
|
pBSII |
pBlueScript II KS(+) |
Ampr
|
Stratagene |
pHP45Ω |
spe cartridge |
Sper
|
31 |
pHP45Ω-Km |
kan cartridge |
Kanr
|
9 |
pSup202 |
Suicide plasmid |
Tetr Cmr Ampr
|
39 |
p4.2 |
cycY::kan in PstI site of pSup202 |
|
This work |
pcycA::spe |
cycA::spe in PstI site of pSup202 |
|
This work |
pKD10.2 |
2-kb spe cartridge of pHP45Ω in BamHI site of cycY carried by pU2000 (in opposite orientation of cycY) |
|
This work |
pU2000 |
1.8-kb BglII-XbaI R. sphaeroides chromosomal DNA carrying cycY in pBSII KS(+) |
|
51 |
pKD11 |
3 kb cycY::spe fragment in EcoRI site of pSup202 |
|
This work |
pSupC2 P2.71::kan |
Δ(cycA::kan) at StuI site of cycA carried at PstI site of pSup202 |
|
8 |