TABLE 3.
Respiratory activities in membranes of various R. sphaeroides mutants grown by respirationa
| Strain | Activity (μmol of O2 consumed/mg of protein/min)
|
|||||||
|---|---|---|---|---|---|---|---|---|
| NADH
|
Succinate
|
Ascorbate
|
||||||
| Alone | +Cyt c | +Myx | +Cyt c, +CN | Alone | +Cyt c | +DCIP | +Cyt c | |
| Ga | 20.6 | 30.9 | 5.8 | 6.2 | 6.9 | 8.3 | 56.0 | 57.0 |
| KD02 | 5.2 | 7.7 | 2.2 | 2.3 | 4.7 | 7.5 | 19.3 | 34.0 |
| KD04 | 2.3 | 4.9 | 1.1 | 1.2 | 2.0 | 6.0 | 3.6 | 43.0 |
| KD03 | 4.8 | 12.0 | 4.1 | 4.4 | 4.8 | 8.8 | 23.0 | 88.0 |
| KD05 | 15.3 | 19.2 | 5.0 | 5.2 | 4.8 | 8.0 | 88.0 | 92.0 |
| JS100 | 9.6 | 11.7 | 0.6 | 0.5 | 4.8 | 7.5 | 44.0 | 35.0 |
| MT001 | 8.9 | 11.1 | 1.2 | 1.1 | 5.2 | 8.8 | 26.0 | 33.0 |
| Gadc2cy | 0.9 | 2.6 | 1.0 | 0.9 | 0.9 | 2.6 | 6.6 | 23.4 |
| Gadc2 | 7.7 | 7.9 | 1.2 | 1.3 | 2.7 | 3.4 | 22.7 | 33.6 |
| Gadcy | 2.0 | 3.6 | 1.4 | 1.3 | 2.3 | 3.7 | 25.4 | 22.2 |
| ME127 | 5.3 | 6.3 | 5.1 | 5.3 | 4.6 | 5.6 | 1.3 | 2.6 |
| BC17 | 3.8 | 3.8 | 3.7 | 3.7 | 3.8 | 3.8 | 39.0 | 41.8 |
a
In these assays, 0.2 mM NADH, 5 mM succinate, 2 mM ascorbate, 0.2 mM DCIP, 50 μM horse heart cyt c, 50 μM CN−, and 2 μM Myx were used as appropriate. Strain genotypes are given in Table 1.