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. 2022 Sep 26;3(4):101773. doi: 10.1016/j.xpro.2022.101773

Figure 3.

Figure 3

Surface expression levels of S protein

(A) Representative FACS data of surface S proteins in HEK293 cells. S protein-expressing-HEK293 cells were harvested at 48 h posttransfection and stained using rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody. Expression levels of the indicated S proteins on the cell surface were measured by flow cytometry and are shown as the MFI in the histogram. Normal rabbit IgG was used as isotype control (gray). pCAGGS was used as the vector. The parent S protein was derived from the B.1.1 variant bearing a D614G mutation.

(B) A summary of S protein expression levels on the HEK293 cell surface. The graph indicates the mean ± standard deviation (SD) MFI (n = 3). pCAGGS was used as a vector. The parent S protein was derived from the B.1.1 variant bearing a D614G mutation. Statistical significance was assessed using two-sided paired t test. ∗ p < 0.05 compared with the parent S protein (blue) and Omicron BA.1 S protein (green).