2 × SDS sample buffer

Reagent	Stock concentration	Final concentration	Amount
Tris-HCl (pH 6.8)	1 M	100 mM	5 mL
SDS	20%	4%	10 mL
Glycerol	100%	20%	10 mL
Bromophenol blue	100%	0.05%	0.025 g
Water	N/A	N/A	up to 50 mL
Total			50 mL