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. 2022 Sep 26;3(4):101773. doi: 10.1016/j.xpro.2022.101773

10 × Tris-buffered saline (TBS)

Reagent Stock concentration Final concentration Amount
Tris N/A 250 mM 30 g
NaCl N/A 1.37 M 80 g
KCl N/A 26.8 mM 2 g
Water N/A N/A up to 1 L
Total 1 L