Table 2.
Fluorophore-conjugated antibody mix
| Target | Fluorophore | Volume per stain | Volume for 20 stains (+10% overage) |
|---|---|---|---|
| IFN-γ | Alexa 700 | 0.2 μL | 4.4 μL |
| Granzyme-B | BV421 | 0.3 μL | 6.6 μL |
| TNF-α | FITC | 0.4 μL | 8.8 μL |
| PD1 | PE | 0.5 μL | 11.0 μL |
| CD45RA | BV605 | 0.5 μL | 11.0 μL |
| CD4 | PE-Cy7 | 0.5 μL | 11.0 μL |
| CD3 | BV785 | 0.6 μL | 13.2 μL |
| IL-2 | PE/Dazzle 594 | 0.7 μL | 15.4 μL |
| Perforin | APC | 0.9 μL | 19.8 μL |
| CD27 | PE-Cy5 | 1.0 μL | 22.0 μL |
| CD8 | BV510 | 1.3 μL | 28.6 μL |
| BD Brilliant Stain Buffer Plus | 10.0 μL | 220 μL | |
| 1× eBioscience Permeabilization solution (q.s 50 μL) | 33.1 μL | 728.2 μL |