Table 5.
The obtained physicochemical properties.
| Characteristics | Finding | Remark |
|---|---|---|
| Number of amino acids | 275 | Suitable |
| Molecular weight | 30.95 kDa | Average |
| Theoretical pI | 9.82 | Basic |
| Chemical formula | C1422H2134N386O383S5 | – |
| Extinction coefficient (at 280 nm in H2O) | 58,790 M−1 cm−1 | – |
| Estimated half-life (mammalian reticulocytes, in vitro) | 30 h | – |
| Estimated half-life (yeast-cells, in vivo) | > 20 h | – |
| Estimated half-life (E. coli, in vivo) | > 10 h | – |
| Instability index of vaccine | 39.84 | Stable |
| Aliphatic index of vaccine | 65.75 | Thermostable |
| Grand average of hydropathicity (GRAVY) | − 0.467 | Hydrophilic |