TABLE 2.

Reporter gene activities from dual transcriptional fusion vector

Plasmida	Mutationb	Alkaline phosphatase activityc		β-Galactosidase activityc
		+ Fe	− Fe	+ Fe	− Fe
pUJ10d	No insert	7	10	0	0
pFD43-1e	Wild type	722	1,898	3	11
pFD43-2	fepD P1 −10	331	996	3	14
pFD43-4	ybdA P1 −10	1,044	2,411	0	0
pFD43-6	Both −10	275	915	0	0
pFD43-30	ybdA P1 “extended −10”	1,052	1,883	1	1
pFD43-31	fepD P1 “extended −10”	341	793	5	20
pFD43-32	fepD P2 −10	1,402	2,518	4	11
pFD43-33	fepD P1 −10 and P2 −35	98	143	2	4
pFD43-3	fepD P1 −35 (up)	6,162	8,773	6	11
pFD43-5	ybdA P1 −35 (up)	1,028	1,398	30	34
PFD43-34	ybdA P1 −35 (up)	1,222	1,644	36	57
pFD43-7	FBS 1	2,730	3,347	15	28
pFD43-1Rf	Wild type	582	1,550	5	21
pFD43-2R	ybdA P1 −10	23	43	6	27
pFD43-6R	Both −10	20	32	1	6

^aPlasmids designated pFD43 contain the 327-bp fepD-ybdA control region in the vector pUJ10, with fepD fused to phoA and ybdA fused to lacZ. The suffix -1 designates the wild-type control sequence. All other suffixes represent the corresponding mutants described in Fig. 3 to 5. The last three plasmids represent the corresponding promoter fragments in the reverse orientation in pUJ10, with -1R containing the wild-type sequence, -2R containing the -2 mutant sequence, and -6R containing the -6 mutant sequence. 

^bBase substitutions for each mutation are described in the legends to Fig. 3 to 5. 

^cActivity is measured in Miller units. 

^dThere was no promoter for pUJ10 when alkaline phosphatase and β-galactosidase activity was measured. 

^eFor this group of plasmids, the promoter when alkaline phosphatase activity was measured was fepD. The promoter when β-galactosidase activity was measured was ybdA. 

^fFor this group, the alkaline phosphatase and β-galactosidase promoters were ybdA and fepD, respectively.