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Monensin titers and analog ratios for various S. cinnamonensis mutants in carbohydrate-based fermentation medium. (A) C730.1, wild-type strain; B, meaA mutant (WM2); C, icm meaA mutant (WD2); D, WM2/pZR8 (plasmid-based expression of meaA); E, WD2/pZR8. Total monensin titers (monensins A and B) are expressed as a percentage of that obtained using the C730.1 strain. The same pattern of changes in ratios and titers was observed using the oil-based media.
