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. 2022 Sep 12;13:827786. doi: 10.3389/fimmu.2022.827786

Figure 3.

Figure 3

EC-induced CD69 expression is most pronounced in effector memory CD8+ T cells. HMEC were stimulated with 10 ng/mL TNFα and IFNγ for 3 days before addition of FACS-sorted naive CD3+ or memory CD3+ T cells to the co-culture. CD69 expression was assessed at various time points of the co-culture by flow cytometry. (A) Percentage of CD69+ cells (left panel) and median fluorescent intensity (MFI) of CD69 (right panel) on naive and memory CD4+ and CD8+ T cells. N = 4, mean+SEM. 2-Way-ANOVA with Sidak post-hoc test. */#P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001. (B) Percentage of CD69+ cells (left panel) and median fluorescent intensity (MFI) of CD69 expression (right panel) on sorted CD8+ memory T cell subsets after co-culture with activated HMEC. TEMRA, terminally differentiated CD45RA+ effector memory T cells (CD45RA+CCR7-); CM, central memory T cells (CD45RA-CCR7+); EM, effector memory T cells (CD45RA-CCR7-). N = 6, mean+SEM. 2-Way-ANOVA with Sidak post-hoc test.