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. 2022 Sep 12;14:973068. doi: 10.3389/fnagi.2022.973068

Figure 7.

Figure 7

Pathological signatures induced by laparotomy were determined by PKR activity. (A) Representative morphological changes of the intestine by H&E staining following laparotomy in the wild-type and PKR−/− mice. (B) Mitochondria membrane potentiation was detected by JC-1 assay in the isolated mitochondria fractions. F(3, 10) = 18.96, P = 0.0002. (C) Representative Western blots of the mitochondria OXPHOS complex in the hippocampi after laparotomy in the wild-type and PKR−/− mice. (D) Western blots of synaptic proteins in the isolated synaptosome from postoperative hippocampi in the wild-type and PKR−/− mice. (E) Relative expressions of autophagy proteins, LC3B: F(3, 16) = 12.58, P = 0.0002; P62: F(3, 16) = 21.14, P < 0.0001; Pink1: F(3, 16) = 17.26, P < 0.0001; Parkin: F(3, 16) = 43.14, P < 0.0001. (F) Relative activities of PKC induced by laparotomy in the wild-type and PKR−/− mice. Wt, wild type; Ctrl, control; Lap, laparotomy. Data are presented as mean ± SEM and were analyzed using one-way ANOVA using Tukey's post hoc test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.