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. 2022 Sep 12;9:1003282. doi: 10.3389/fcvm.2022.1003282

FIGURE 5.

FIGURE 5

Adipsin-enriched pericardial-AT exosomes regulate iron homeostasis and alleviate lipid oxidative stress in vivo. (A,B) Non-heme iron levels in the serum and the peri-infarct region of the cardiac tissue were assessed in MI mouse after treatment with Adipsin-transgenic or non-transgenic exosomes. Each group n = 6, *p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, Ip < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, §p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (C) The levels of malondialdehyde (MDA) in cardiac tissue were measured in the peri-infarct area. Each group n = 6, *p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg mice, Ip < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg mice, §p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg mice. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis. (D) Perls’ Prussian Blue-stained heart slices. (E–I) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6, *p < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-NTg, Ip < 0.05 vs. Sham + Pericardial-AT exosomes from Adipsin-Tg, §p < 0.05 vs. MI + Pericardial-AT exosomes from Adipsin-NTg. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.