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. 2022 Sep 12;9:1003282. doi: 10.3389/fcvm.2022.1003282

FIGURE 7.

FIGURE 7

Irp2 is essential to Adipsin-offered cardioprotection. (A) Flowchart of the study design. Myocardial infarction surgery was performed 14 days after injection of AAV9 (Scramble or IRP2-shRNA) at the area of the myocardium. Following that, MI mice were injected four times with exosomes isolated from Adipsin-Tg or NTg mice’s pericardial adipose tissues. Cardiac function was assessed 28 days after MI surgery. Each group n = 6. (B,C) Representative echocardiographic images and left ventricular ejection fraction (LVEF) analysis. Each group n = 6. *p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (D) Non-heme iron levels were measured in the peri-infarct region of heart. Each group n = 6. *p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (E,F) Quantification of fibrotic area and representative images of Masson’s trichrome staining of the transverse planes post myocardial infarction. Each group n = 6. *p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (G) Malondialdehyde (MDA) levels in the peri-infarct region of heart. Each group n = 6. *p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. (H–L) TFRC, FTH, COX2, and GPX4 proteins were measured by Western blots. Each group n = 6. *p < 0.05 vs. AAV9-Scramble + Exo (NTg) group. Data were mean ± SEM, and one-way ANOVA was used for statistical analysis.