FIGURE 2.

Effect of 1j on LPS-induced c-Jun (Ser63) phosphorylation. Human MonoMac-6 monocytic cells were pretreated with the indicated concentrations of compounds or 0.5% DMSO for 30 min, followed by treatment with LPS (250 ng/ml) or control buffer for another 30 min. Controls with cells alone (no DMSO) or cells treated with LPS alone were also included. The cells were lysed, and the lysates were analyzed by Western blotting on 10% SDS-PAGE gels. A representative blot from three independent experiments is shown (A). The blot was analyzed by densitometry as described under Materials and Methods, and the ratio of phospho-c-Jun/total c-Jun is shown in (B). Values are expressed as mean ± SD of three independent experiments.