FIGURE 3.

Effect of the compound 1j on proinflammatory cytokine production in MonoMac-6 cells. Human MonoMac-6 monocytic cells were pretreated with 10 μM of 1j or DMSO for 30 min, followed by addition of 250 ng/ml LPS or buffer for 24 h. Production of cytokines in the supernatants was evaluated using a Multiplex human cytokine ELISA kit. The relative level of cytokine production is shown as fold increase over background (i.e., 1% DMSO control). The data are presented as the mean ± S.D. of triplicate samples from one experiment that is representative of two independent experiments.