FIG. 2.
The 52-bp sequence containing the oriT and the promoter of the mob gene (coordinates 918 to 867). Horizontal arrows indicate inverted repeats. The vertical arrow indicates the position of the nick site. Boxes marked −10 and −35, proposed regions for the promoter of the mob gene. The 52-bp sequence was cloned in vector pKilpCR2, yielding poriT52bp. The Bst1107 site used to construct poriT18bp and poriT34bp is indicated. Mobilization frequencies were determined by matings between Nals B462 donors (4) containing pBBR1CM (Mob donor), the conjugative IncP plasmid pSL2T, and the poriT plasmid to be tested and Nalr B462 recipients. Shaded sequence, RSA region as defined for the reference plasmid pMV158.