FIG. 3.

Overproduction of the Mob protein. One microliter of crude extract of BL21(DE3, pLys) containing the appropriate plasmid was electrophoresed on SDS–10% polyacrylamide gels. The gels were stained with Coomassie brilliant blue. Lane 1, molecular mass marker (masses in kilodaltons are shown on the left); lane 2, 1 μl of crude extract of 15 ml of BL21(DE3, pLys, pETMob) without induction; lane 3, 1 μl of crude extract of 15 ml of BL21(DE3, pLys, pETMob) with induction; lane 4, 1 μl of crude extract of 15 ml of BL21(DE3, pLys, pETMob-GFP); lane 5, 1 μl of crude extract of 15 ml of BL21(DE3, pLys, pETLacZ).