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. 2021 Feb 23;20(1):101–109. doi: 10.1016/j.gpb.2020.06.017

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Supplementary Figure S1 — Characterization of the 2D- and 3D-cultured AML12 cells A. Flow cytometry of the synchronized 2D- and 3D-cultured cells. B. and C. Confocal images of the nuclei of 2D-cultured (B) and 3D-cultured (C) cells stained with DAPI (blue) and lamina B (red). D. and E. Confocal images of 2D-cultured (D) and 3D-cultured (E) cells stained with DAPI (blue) and Wheat Germ Agglutinin (red to label the cell membrane). F. Digestion and ligation efficiency before and after optimization of the conditions to culture cells in 3D.