Figure 1.

Regulation and enrichment of mRBPs in failing human hearts

A. Regulation of 421 mRPB-coding genes in the heart of patients diagnosed with DCM (n = 33) compared to controls (n = 24) (Student’s t-test). Size of the bubble indicates a HSS (arbitrary unit) in 53 different human tissues from a total of 11,467 human tissue samples. B.SLM2 mRNA expression levels in heart tissues of patients (DCM and ICM; each n = 3) and controls (n = 5). The SLM2 mRNA expression levels were normalized to the mRNA expression level of RPLP0, which served as the reference gene. C. Representative IB image depicting the protein expression levels of SLM2 in heart tissues of DCM patients and controls. α-actin served as a loading control. D. Densitometric quantification of SLM2 protein expression in heart tissues of DCM patients (n = 4) and controls (n = 3). α-actin served as a loading control and was used for normalization. *, P < 0.05 (Student’s t-test). mRPB, mRNA-binding protein; DCM, dilated cardiomyopathy; ICM, ischemic cardiomyopathy; HSS, heart-specificity score; SLM2, Sam68-like mammalian protein 2; IB, immunoblot.