Table 3.
Validation strategy | Total | Valid | Invalid | Inconclusive | |
---|---|---|---|---|---|
Experimental (PCR succeeded) | 21 | 15 (71%) | 6 (29%) | – | |
Computational |
ONT reads | 609 | 256 (42%) | – | 353 (58%) |
HiFi contigs | 414 (68%) | 195 (32%) | – | ||
ONT reads or HiFi contigs | 533 (87%) | 76 (13%) | – | ||
Manual | HiFi reads | 609 | 440 (72%) | 169 (28%) | – |
Note: ONT, Oxford Nanopore Technologies; HiFi, Pacific Biosciences high-fidelity.