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. 2021 Jul 3;20(1):205–218. doi: 10.1016/j.gpb.2021.03.007

Table 3.

Summary of experimental and computational validations as well as manual inspections for CSVs

Validation strategy Total Valid Invalid Inconclusive
Experimental (PCR succeeded) 21 15 (71%) 6 (29%)
Computational
ONT reads 609 256 (42%) 353 (58%)
HiFi contigs 414 (68%) 195 (32%)
ONT reads or HiFi contigs 533 (87%) 76 (13%)
Manual HiFi reads 609 440 (72%) 169 (28%)

Note: ONT, Oxford Nanopore Technologies; HiFi, Pacific Biosciences high-fidelity.