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. 2022 Sep 12;13:936159. doi: 10.3389/fendo.2022.936159

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Copyright © 2022 Ballato, Deepika, Prado, Russo, Fuenmayor, Bathina, Villareal, Qualls and Armamento-Villareal

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Gating Strategy for Circulating Osteogenic Progenitor Cell Isolation. Cells are first sorted along forward/side scatter to identufy nucleated cells, then forward area and height to identify singlets, then DAPI-negative cells are identified as viable. Cells that are CD3-CD19-CD56-are gated from this population the OCN+ cells are identified as osteoblast progenitors. (B) Gating Strategy for Osteoclast Precursor Cell Isolation. Cells are first sorted along forward/side scatter to idfentify nucleated cells, then forward area and height to identify singlets, then DAPI-negative cells are identified as viable. Cells that are CD14+CD11b+, CD14+MCSFR+, or CD14+CD120b+ are identified as OC precursors.