Figure 4.

No proteolytic action of M101 on C3 to C9 fractions. EDTA whole blood from healthy controls was pre-incubated with increasing amounts of M101 (buffer, 0.5 g/L, 1 g/L and 5 g/L) during 12h, 24h and 48h before being centrifuged and EDTA-plasma frozen (-80°C). C3 and C4 fractions were quantified by turbidimetry (n=10), results are expressed as g/L, while radial immunodiffusion (n=3) was used for the fractions C5 to C9 and results were expressed as the diameter of the ring (mm). Statistical analysis did not reach significance.