TABLE 2.

Determination of DCH quantification with degree of hepatitis, proliferative and apoptotic activities of hepatocytes in infected cats

Sample No.	Viral copy number (/μL)	Histology	Inflammation grade a		Vacuolation score	ISH b	PCNA	Survivin	Phospho histone‐H3	Caspase‐3	TUNEL
			Portal area	Parenchymal area
1	4.33 × 103	HNL	0	0	2	Rare	−	+	−	+	+
2	1.74 × 108	HPD; C, I	3	6	3	Widespread	+++	+++	++	−	−
3	4.35 × 106	HPD; C, I	2	4	2	Widespread	++	+++	+	−	−
4	2.38 × 104	HPD; A	1	1	1	Rare	+	−	+	−	−
5	1.65 × 102	HNL	0	0	3	No signals	−	−	−	+	+
6	4.35 × 104	HPD; A	1	1	2	Rare	+	−	−	−	−
7	1.05 × 105	HPD; C	1	1	0	Common	+	++	+	−	−
8	1.22 × 105	HPD; C	1	3	5	Common	+	+	−	−	−
9	2.65 × 109	HPD; C, I	3	6	4	Widespread	+++	+++	++	−	−
10	1.33 × 105	HPD; C, I	3	6	2	Widespread	+++	+++	++	−	−
11	2.65 × 104	HPD; C	2	4	5	Common	++	+++	++	−	−

Abbreviations: Averaged positive immunoreactivity of proliferative/apoptotic indices: −, no immunoreactivity; +, ≤25% positive immunoreactivity; ++, 26%‐50% positive immunoreactivity; +++, ≥50% positive immunoreactivity. A, acute hepatitis; C, chronic hepatitis; HNL, histologically normal liver; HPD, histological parenchymal disorder; I, interface hepatitis; ISH, in situ hybridization; PCNA, proliferating cell nuclear antigen; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.

^a Significant association between viral copy number and histological grading of portal inflammation (P = .014) and parenchymal inflammation (P = .03).

^b Significant correlation between viral copy number and intensity of staining pattern (P = .004).