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. 2022 Sep 12;13:974688. doi: 10.3389/fphys.2022.974688

TABLE 1.

Oligonucleotide primer sequences used for RT-qPCR.

Tissue  Gene Primer F Primer R References
  GAPDH ACG​GAT​TTG​GTC​GTA​TTG​GG TGA​TTT​TGG​AGG​ATC​TCG​C Grandhaye et al. (2021)
EEF1α AGC​AGA​CTT​TGT​GAC​CTT​GCC TCA​CAT​GAG​ACA​GAC​GGT​TGC
β-actin ACG​GAA​CCA​CAG​TTT​ATC​ATC GTC​CCA​GTC​TTC​AAC​TAT​ACC
Liver CYP1A1 AAT​GCT​CGT​TTC​AGT​GCC​TTC CCT​CCC​CTG​TCC​TTT​TCT​CC Cong et al. (2019)
CYP1A2 AAC​CCA​GAG​CGT​TTC​CTC​AA CTC​CCA​CTT​GCC​TAT​GTT​TTC​C
CYP2C CAA​AAT​GGA​ACA​GGA​GAA​AGA​GAA​C CCC​GCA​AGG​AAC​AAG​TCA​A
CYP3A CCA​AGC​TAT​GCT​CTT​CAC​CG TCA​GGC​TCC​ACT​TAC​GGT​CT
CYP2A6 CTG​CAG​AGA​ATG​GCA​TGA​AG CCT​GCA​AGA​CTG​CAA​GGA​A Wang et al. (2018)
EPHX1 GAA​GAT​GTC​AGG​CGG​ATG​TT CAG​GAG​AGT​CAT​TCA​AAC​CAC​A
GSTA3 AGA​CCA​GAG​CCA​TCC​TCA​AC TGC​CAG​TCC​TTC​CAC​ATA​CA
GSTA4 GCT​ACA​TCG​CAG​GGA​AAT​ACA TGG​AGA​GAA​AGG​AAA​CAC​CAA
FXR AAAGCCGTTCTGTGCGTT GGATTGGTGGGGTTCCTG Dai et al. (2020)
CYP3A37 AAA​TCA​GAC​AGC​AAT​GGG​AGC GGT​AAG​CCA​GGT​AAC​CAA​GTG​T
BSEP TGCAAAGCAAAGGAGACT GCAATGGATAATGGAGGG
CYP1A5 CTCTGCTCTGTTCDCAAAGCGTCTC GCTCGCCTGCDCACCDCATCDCACT
CYP1A4 CDCAGGACGGAGGCTGACDCAAGG GCDCAGGATGGTGGTGAGGAAGA
Ces1 TGACCATTCAATATCGCC ACACTTTCTCCTCCCGCT
SLC O 1B3 CAG​GAC​TCT​CGC​TGG​GTG​G TGG​CTT​TCA​GGG​GCT​TTT​T
CYP2H1 TCA​TCC​ACG​AAA​TCC​AAA​G GATGGGAGACAGCAAAGG
CYP2H2 GGCCCGGATGGAGCTATT TTGCCGCCGAGGTGACTA
Gizzard Hsp27 ACA​CGA​GGA​GAA​ACA​GGA​TGA​G ACTGGATGGCTGGCTTGG Zhao et al. (2016)
Hsp40 GGG​CAT​TCA​ACA​GCA​TAG​A TTC​ACA​TCC​CCA​AGT​TTA​GG
Hsp60 AGCCAAAGGGCAGAAATG TAC​AGC​AAC​AAC​CTG​AAG​ACC
Hsp70 CGGGCAAGTTTGACCTAA TTG​GCT​CCC​ACC​CTA​TCT​CT
Hsp90 TCC​TGT​CCT​GGC​TTT​AGT​TT AGGTGGCATCTCCTCGGT
NF-κB TCA​ACG​CAG​GAC​CTA​AAG​ACA​T GCA​GAT​AGC​CAA​GTT​CAG​GAT​G Xing et al. (2015)
iNOS CCT​GGA​GGT​CCT​GGA​AGA​GT CCT​GGG​TTT​CAG​AAG​TGG​C
COX-2 TGT​CCT​TTC​ACT​GCT​TTC​CAT TTC​CAT​TGC​TGT​GTT​TGA​GGT
PTGES GTT​CCT​GTC​ATT​CGC​CTT​CTA​C CGC​ATC​CTC​TGG​GTT​AGC​A
TNF-α GCC​CTT​CCT​GTA​ACC​AGA​TG ACA​CGA​CAG​CCA​AGT​CAA​CG
Proventriculus PGA5 TCC​GTC​TAC​CTG​AGC​AAG​GAT AAG​CAG​GCG​ACG​TAC​TTG​TT Al-Khalaifah et al. (2020)
PGC ATC​GGG​ATT​GAG​GAC​TTC​GC TGA​AGA​CCT​GGT​TGG​GAA​CG
Caecum Chemerin CGCGTGGTGAAGGATGTG CGA​CTG​CTC​CCT​AAA​GAG​GAA​CT Estienne et al. (2020)
CMKLR1 CGGTCAACGCCATTTGGT GGG​TAG​GAA​GAT​GTT​GAA​GGA​A
IgA GTC​ACC​GTC​ACC​TGG​ACT​ACA ACC​GAT​GGT​CTC​CTT​CAC​ATC Grandhaye et al. (2020)
IFNα CAA​CGA​CAC​CAT​CCT​GGA​CA GGG​CTG​CTG​AGG​ATT​TTG​AA Garrido et al. (2018)
IFNβ TCC​TGC​AAC​CAT​CTT​CGT​CA CAC​GTC​TTG​TTG​TGG​GCA​AG
IL-1β AGG​CTC​AAC​ATT​GCG​CTG​TA CTT​GTA​GCC​CTT​GAT​GCC​CA Grandhaye et al. (2020)
IL-6 GCT​TCG​ACG​AGG​AGA​AAT​GC GCC​AGG​TGC​TTT​GTG​CTG​TA Garrido et al. (2018)
IL-8 CTG​CGG​TGC​CAG​TGC​ATT​AG AGC​ACA​CCT​CTC​TTC​CAT​CC
Spleen IFNα CAA​CGA​CAC​CAT​CCT​GGA​CA GGG​CTG​CTG​AGG​ATT​TTG​AA Garrido et al. (2018)
IFNβ TCC​TGC​AAC​CAT​CTT​CGT​CA CAC​GTC​TTG​TTG​TGG​GCA​AG
IL-1β AGG​CTC​AAC​ATT​GCG​CTG​TA CTT​GTA​GCC​CTT​GAT​GCC​CA Grandhaye et al. (2020)
IL-8 CTG​CGG​TGC​CAG​TGC​ATT​AG AGC​ACA​CCT​CTC​TTC​CAT​CC Garrido et al. (2018)
IL-22 TGT​TGT​TGC​TGT​TTC​CCT​CTT​C CAC​CCC​TGT​CCC​TTT​TGG​A