TABLE 4.

Relative mRNA expression of various genes in hen’s organs at the end of GBH exposure (D42, CT, n = 9 and GBH, n = 9) hens) and after dietary GBH exposure (D70, Ex-CT, n = 12 and Ex-GBH, n = 12 hens).

Organ		D42			D70
		CT	GBH	p.value	Ex-CT	Ex-GBH	p.value
Liver	CYP1A2	10.47	8.74	0.578	16.42	12.31	0.210
	CYP1A4	0.110	0.130	0.598	0.170	0.190	0.835
	CYP2A6	20.0	18.4	0.696	15.1	16.28	0.741
	CYP2C	11.4	9.86	0.498	12.4	11.40	0.793
	CYP3A37	6.87	6.78	0.956	5.11	5.16	0.966
	CYP3A4	0.300	0.35	0.401	0.35	0.310	0.425
	CYP3A80	0.460	0.42	0.681	1.98	0.740*	0.027
	CYP2H1	22.8	22.0	0.854	16.7	18.4	0.661
	CYP2H2	13.0	13.44	0.905	10.2	12.4	0.432
	GSTA3	3.10	2.92	0.700	5.65	3.86	0.200
	GSTA4	4.71	3.50	0.054	4.43	4.32	0.861
	EPHX1	0.500	0.45	0.541	0.40	0.44	0.763
	FXR	0.760	0.78	0.881	1.49	1.24	0.461
	SL O 1B3	0.710	0.71	0.972	0.71	0.77	0.759
Gizzard	COX2	0.201	0.132	0.188	0.188	0.142	0.194
	HSP27	39.9	33.8	0.637	64.1	53.4	0.360
	HSP40	94.1	93.1	0.835	91.3	93.3	0.582
	HSP60	0.533	0.361	0.113	0.707	0.628	0.310
	HSP70	3.18	2.48	0.443	4.31	2.29*	0.015
	HSP90	2.24	1.49	0.249	1.87	1.55	0.544
	iNOS	0.028	0.022	0.412	0.023	0.042**	0.003
	NFκB	0.079	0.059	0.417	0.104	0.099	0.760
	PTGES	0.087	0.054	0.168	0.074	0.055	0.245
	TNFa	0.036	0.029	0.154	0.023	0.033	0.052
Proventriculus	PGA5	32,266	38,158	0.330	26,653	27,223	0.899
	PGC	5,916	7,495	0.114	6,874	6,838	0.971
Caecum	Chemerin	0.336	0.337	0.986	0.272	0.257	0.731
	CMKLR1	0.017	0.017	0.984	0.065	0.048	0.301
	IgA	12.3	8.49	0.364	4.46	6.70*	0.041
	IFNα	0.071	0.086	0.620	0.434	0.290	0.230
	IFNβ	0.002	0.005	0.179	0.024	0.011	0.100
	IL-1β	0.013	0.007	0.095	0.020	0.016	0.338
	IL-6	0.001	0.001	0.895	0.004	0.002	0.077
	IL-8	0.040	0.025	0.185	0.049	0.032	0.242
Spleen	IFNα	0.031	0.025	0.607	0.112	0.061	0.108
	IFNβ	3.50	2.18	0.301	7.90	6.00	0.414
	IL-1β	0.006	0.004	0.182	0.010	0.014	0.279
	IL-8	0.076	0.075	0.964	0.065	0.038*	0.036
	IL-22	0.035	0.021	0.273	0.093	0.024**	0.003

Results are presented as mean of the pltarget gene mRNA, expression relative to the geometric mean of three housekeeping genes expression (GAPDH, EEF1α and β-actin); *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. CT, control; GBH, Ex-CT, Ex-Control, Ex-GBH .