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. 2022 Sep 12;13:984302. doi: 10.3389/fimmu.2022.984302

Figure 2.

Figure 2

miR-146aKO B cells have higher in vitro response to T-independent antigens than WT and demonstrate increased antibody secretion in response to stimulation. (A, B) Stimulation of WT native splenic B-cells with (A) LPS or (B) CpG shows significant miR-146a induction at time points 72 and 96 hr (one-way ANOVA with Tukey’s multiple-comparisons test; ***P < 0.001). Graph combines three independent experiments. n = 3-4 mice/group/exp. Data represent mean ± SEM. (C and D) miR-146aKO splenic B-cells stimulated with (C) LPS or (D) CpG have higher cell counts than WT at various time points (t test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Representative of 3-4 mice/group in triplicates, confirmed in at least six independent experiments. (E, F) miR-146aKO marginal zone and follicular B-cells show higher cell count than WT when stimulated with (E) LPS or (F) CpG (t test; ***P < 0.001, ****P < 0.0001). Representative of three mice/group in triplicates, confirmed in three independent experiments. (G) Supernatants from in vitro stimulation at 72hr show higher total IgM secretion in miR-146aKO B cells compared to WT.