Figure 7.

The effect of TGFβ inhibitor A83-01 on microvessels: (a) the primary microvessels were cultured in the media supplemented with different concentrations of A83-01, including 0, 0.1, 0.2, 0.5, 1, 2 μM, for 2 weeks, and immunostained by the antibodies against CD31 and NG2. Nuclei were stained by DAPI. Scale bars, 100 μm. (b and c) quantification of microvessel length and branch point number in different groups. Data were presented as mean ± SD. One-way ANOVA was performed on the data, followed by Bonferroni post hoc tests. *p < 0.05, **p < 0.01.