Figure 8.

Dynamic regulation of NG2 pericytes by A83-01: (a–c) the primary microvessels were cultured in CY for 14 days (a), in CAY for 14 days (b), and in CAY for 7 days, followed by CY for another 7 days (c), then immunostained by antibodies against CD31 and NG2. Nuclei were stained by DAPI. Scale bars, 100 μm. (d) quantification of microvessel length, NG2+ pericyte number per microvessel length, and NG2+ pericyte coverage ratio of total microvessel length (%). Data were presented as mean ± SD. Student’s t-test was performed to analyze significant differences between groups. ***p < 0.001.