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Experimental layout to study TCM, TTM, and the mechanism of their action in vitro using HCC and CCA cell lines. (A) Cells were seeded on day—1; (B) on day 0 compounds C1, C2 and C3 were added at different concentrations as a monotherapy or in combination with standard chemotherapeutics; (C) after 24 and 48 h different readouts were performed and comprised bright field microscopy, CVSA, CCK-8 and SA-β-Gal assays as well as FACS analysis.
