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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods. 2022 Aug 17;206:58–68. doi: 10.1016/j.ymeth.2022.08.007

Figure 1. RfxCas13d has more efficient target mRNA KD activity than other Cas13 orthologs.

Figure 1.

(A) A flowchart for the workflow of a typical Cas13 RNA KD experiment in mammalian cell cultures. (B) Comparison of KD efficiencies of different Cas13 proteins targeting the same region of KRAS mRNA. 100 ng of Cas13 expressing vector were co-transfected into HEK293T cells with 100 ng of corresponding gRNA expressing vector for 8 hours before media change and harvested to measure KRAS mRNA expression levels by RT-qPCR (n>3 replicates) with GAPDH used as a housekeeping control. Data were normalized to a mock transfection control. NT: non-targeting control gRNA. The error bars represent the mean ± SEM, respectively.