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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods. 2022 Aug 17;206:58–68. doi: 10.1016/j.ymeth.2022.08.007

Figure 2. Determining the optimal conditions for co-transfecting RfxCas13d and gRNA expression plasmids in HEK293T cells.

Figure 2.

(A) The magnitude of target gene KD targeting the same region of KRAS mRNA as determined by RT-qPCR, as a function of total DNA transfected (n>3 replicates). GAPDH was used as a housekeeping control. Data were normalized to a mock transfection control. The error bars represent the mean ± SEM, respectively. (B) The magnitude of target gene KD targeting the same region of KRAS mRNA as determined by RT-qPCR, as a function of the time the cells were exposed to transfection reagent (n>3 replicates). Data were normalized to a mock transfection control. NT: non-targeting control gRNA. The error bars represent the mean ± SEM, respectively.