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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods. 2022 Aug 17;206:58–68. doi: 10.1016/j.ymeth.2022.08.007

Figure 4. Increasing length of gRNA increases KD performance.

Figure 4.

(A) The magnitude of target gene KD targeting KRAS mRNA and with the specified gRNAs containing either 22-nt. or 30-nt. gRNA-spacers, as determined by RT-qPCR (n>3 replicates). GAPDH was used as a housekeeping control. Data were normalized to a mock transfection control. The error bars represent the mean ± SEM, respectively.