Table 2.
gRNA expression plasmids, sequences, and genes targeted
| gRNA expression plasmid | gRNA Sequence (5’-3’) | Gene targeted | gRNA | Figure |
|---|---|---|---|---|
| pC0041 | GTATTATTTATGGCAAATACACAAAGAAAG | KRAS | 9 | 1B |
| pC0041 | GCAGGGTTTTCCCAGTCACGACGTTGTAAAA | NT | NT | 1B |
| pC0043 | GTATTATTTATGGCAAATACACAAAGAAAG | KRAS | 9 | 1B |
| pC0043 | GCAGGGTTTTCCCAGTCACGACGTTGTAAAA | NT | NT | 1B |
| pXR003 | CTATAATGGTGAATATCTTCAAATGATTTA | KRAS | K7 | 4A |
| pXR003 | TCAGAGTCCTTAACTCTTTTAATTTGTTCT | KRAS | K8 | 1B, 2B, 2C, 4A, S1C, S2C |
| pXR003 | TAATTTGTTCTCTATAATGGTGAATATCTT | KRAS | K9 | 4A |
| pXR003 | TCACCAGAAGCGTACCATACTCACGAACAG | NT30 | NT | 1B, 2B, 2C, 3B, 4B, 4C, S1A, S1B, S2A, S2B |
| pXR003 | GGTAATGCCTGGCTTGTCGACGCATAGTCT | NT57 | NT | 4B,4C |
| pXR003 | CAGACAATTGTGTCGCTGCCATC | CD46 | 1 | 3A, 3B, 3C, 3D |
| pXR003 | ACAATTGTGTCGCTGCCATCGAG | CD46 | 2 | 3A, 3B, 3C, 3D |
| pXR003 | TCATTACAAATAAAGTGCATCTG | CD46 | 3 | 3A, 3C, 3D |
| pXR003 | CCAGAAGCAACCCAGGAAAGCGC | CD46 | 4 | 3A, 3C, 3D |
| pXR003 | TGATCCTTTAAGTTCACAATATA | CD46 | 5 | 3A, 3C, 3D |
| pXR003 | CTGAATTGTCACCACAATAAATC | CD46 | 6 | 3A, 3C, 3D |
| pXR003 | TCAAAAGATGAACTGGCAAACCA | CD46 | 7 | 3A, 3C, 3D |
| pXR003 | TTGCAATATTAGCTAAGCCACAG | CD46 | 8 | 3A, 3C, 3D |
| pXR003 | TTAGAGAATTTTCCAATAATATT | CD46 | 9 | 3A, 3C, 3D |
| pXR003 | TAATTTTGCCAATTTTATAAAAT | CD46 | 10 | 3A, 3C, 3D |
| pXR003 | AGACAATTGTGTCGCTGCCATCG | CD46 | 11 | 3A, 3B, 3C, 3D |
| pXR003 | ATAACAATCACAGCAATGACCCA | CD46 | 12 | 3A, 3C, 3D |
| pXR003 | TCACAAATAGTATGGGTGGCAAG | CD46 | 13 | 3A, 3C, 3D |
| pXR003 | GCGGCGGCCGGGAGGCTCCATGC | CD46 | 14 | 3A, 3C, 3D |
| pXR003 | AACTCGTAAGTCCCATTTGCAGG | CD46 | 15 | 3A, 3C, 3D |
| pXR003 | ATGTGCCTTTCTTCTTCCTCCTT | CD46 | 16 | 3A, 3C, 3D |