Table 3.
Forward Primer (5’ – 3’) | Probe sequence (5’ – 3’) | Reverse Primer (5’ – 3’) | Gene and Probe ID |
---|---|---|---|
CCT ACT AGG ACC ATA GGT ACA TCT | /5FAM/AGT CCT TAA /ZEN/CTC TTT TAA TTT GTT CTC TAT AAT GGT GAA T/3IABkFQ/ | AGG GCT TTC TTT GTG TAT TTG C | KRAS (Hs.PT.58.41104810) |
TGT AGT TGA GGT CAA TGA AGG G | /5Cy5/AAG GTC GGA GTC AAC GGA TTT GGT C/3IAbRQSp/ | ACA TCG CTC AGA CAC CAT G | GAPDH (Hs.PT.39a.22214836) |