Component | Volume/Reaction (μl) |
---|---|
10X RT Buffer | 0.5 |
25X dNTP Mix (100 mM) | 0.2 |
10X random hexamers | 0.5 |
Multiscribe Reverse Transcriptase | 0.25 |
Nuclease-free Water | 1.05 |
Total per Reaction | 2.5 |
Component | Volume/Reaction (μl) |
---|---|
10X RT Buffer | 0.5 |
25X dNTP Mix (100 mM) | 0.2 |
10X random hexamers | 0.5 |
Multiscribe Reverse Transcriptase | 0.25 |
Nuclease-free Water | 1.05 |
Total per Reaction | 2.5 |