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. 2022 Sep 26;21:41. doi: 10.1186/s12941-022-00533-3

Table 1.

Oligonucleotide sequences used in this study

Target gene Primer designation Oligonucleotide sequence (5′-3′) PCR conditions Reference
BCL-2 BCL-2 F: GAGCTGGTGGTTGACTTTCTC 95 °C 10 min, 40 cycles (95 °C 10 s; 56 °C 35 s; 72 °C 20 s), 72 °C 5 min [48]
BCL-2 R: TCCATCTCCGATTCAGTCCCT
TNF-α TNF-α F: AGCCCATGTTGTAGCAAACC [49]
TNF-α R: TGAGGTACAGGCCCTCTGAT
TGF-β TGF-B F: CAATTCCTGGCGATACCTCAG [50]
TGF-B R: GCACAACTCCGGTGACATCAA
NF-kB NF-kB F: CCAGACCAACAACAACCCCT [51]
NF-kB R: TCACTCGGCAGATCTTGAGC
SMAD3 SMAD3 F: GCCTGTGCTGGAACATCATC [52]
SMAD3 R: TTGCCCTCATGTGTGCTCTT
β-actin ACTBF F: ATGTGGCCGAGGACTTTGATT [53]
ACTBF R: AGTGGGGTGGCTTTTAGGATG