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. 2022 Sep 26;41:284. doi: 10.1186/s13046-022-02493-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — AADAC reduces lipid peroxidation. A Significantly enriched lipid species detected by untargeted lipidomic analysis in sh-AADAC HCT116 cells compared to shNC cells. B Heatmap of PUFA-containing PEs significantly enriched in sh-AADAC HCT116 cells. C Representative ROS levels in shNC and sh-AADAC HCT116 cells. D Representative images and quantification of lipid droplets in control, sh-AADAC, and AADAC-oe (ADOE) HCT116 cells (scale, 100 μm). E Venn diagram of significantly enriched and proferroptotic lipid species among 84 upregulated lipids in sh-AADAC cells. F Fold change, p value, and VIP value of 5 proferroptotic lipid species in (E). Fold change > 1.5; FDR-related p value < 0.05; VIP value of lipidomic analysis > 1. G Relative cellular MDA levels in shNC and sh-AADAC HCT116 cells treated with 15 μM erastin. H Relative levels of GSH, GSSG, and the GSH/GSSG ratio in sh-AADAC HCT116 cells. Data are shown as the mean ± SD. Significance was calculated by a two-tailed ratio t test (right of C, left of D, G-H). p value < 0.001 (***), p value < 0.01 (**), p value < 0,05 (*), ns (not significant)